Detection of human DNA polymorphisms with a simplified denaturing gradient gel electrophoresis technique.
نویسندگان
چکیده
منابع مشابه
Detection of human DNA polymorphisms with a simplified denaturing gradient gel electrophoresis technique.
Single base pair differences between otherwise identical DNA molecules can result in altered melting behavior detectable by denaturing gradient gel electrophoresis. We have developed a simplified procedure for using denaturing gradient gel electrophoresis to detect base pair changes in genomic DNA. Genomic DNA is digested with restriction enzymes and hybridized in solution to labeled single-str...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1987
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.84.10.3339